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Enzymatic reaction mechanism In order for the reaction to occur, any two molecules with the correct orientation and sufficient energy must collide. The energy between these molecules needs to overcome the reaction barriers. This energy is called activation energy. The enzyme requires an active center. The active site is part of a molecule that has a functional group to bind a particular shape to a reactant molecule. Molecules that bind to enzymes are called substrate groups. Substrate and enzyme form an intermediate reaction with low activation energy without catalyst.

A molecule, a set of two or more atoms forming a small, undetectable unit where a pure object can be separated and retains the structure and chemical properties of that object. Molecular characteristics The separation of a sample of an object into smaller parts continuously does not produce a change in its composition or chemistry until the components comprising a single molecule are reached. Further classification of matter leads to smaller residues that are usually different from the original object in its construction and are always different from it in chemical properties. In this final phase of the separation the chemical bonds that hold the atoms together in the molecule break down.